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Semen Analysis

Semen Analysis

The Fertility Center AKESO provides a specially designed sperm collection room aiming to make the process as comfortable as possible for the partner. The instructions below should be considered before beginning semen collection:

a) Semen collection is self-collected via masturbation and ejaculation is collected into an individually wrapped sterile container with a wide opening. 

b) Preferably should be a 2-4 days period of sexual abstinence prior to the collection to maximize the recovery of the number and quality of collected spermatozoa.

c) It is important to collect the entire specimen, especially the first few drops.

d) In case the specimen should be produced at a venue other than the Center it must be delivered to the laboratory within half an hour. The container should be kept warm close to body temperature during the transport, inside either a trouser or a jacket pocket. 

e) You must ensure that the lid of the container is screwed on firmly, and that your name, the time and date of the collection are written on the label.



Semen Analysis

Male infertility can be detected by semen analysis, a test which is performed to evaluate sperm quantity and quality.


This test includes a macroscopic examination and a microscopic investigation of a variety of semen parameters including:

• Liquefaction
• Viscosity
• Appearance
• Agglutination
• Appearance of Cellular elements (millions/ml)
• Volume (ml)
• Concentration (sperm count, millions/ml),
• Motility (percentage of sperm moving)
• Morphology (percentage of normally-shaped sperm)

The reference values (normal characteristics) for the examination of human semen sample are in accordance to the World Health Organization (WHO). Abnormalities in one or more of these parameters might compromise the ability of the sperm to fertilize the eggs and can be described in the following indications:

• Oligozoospermia
• Asthenozoospermia
• Teratozoospermia
• Oligoasthenoteratozoospermia (combination of all the above)
• Azoospermia


Swim-up Method:

An additional test that indicates the ability of spermatozoa to fertilize an egg in vitro, is performed using the Swim-up method. This method allows the spermatozoa to “swim” upwards into a culture medium. This culture medium that is consisted of spermatozoa of the best progression and dynamic of the sample is assessed. Assessment of the final number and dynamic of the spermatozoa is a strong indicator of their fertilization ability in vitro.

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